Paper-chromatographic techniques for hydrazides and certain metabolites was written by Greulach, Victor A.;Haesloop, John G.. And the article was included in Anal. Chem. in 1961.Quality Control of 1,2-Dihydro-4-methyl-3,6-pyridazinedione This article mentions the following:
A large number of hydrazides were separated by descending chromatography by using either 7:1:2 iso-PrOH:NH4OH:H2O or 5:1:4 BuOH:HOAc:H2O. Alternatively, 2-dimensional separation could be used, developing with each solvent in turn. Spots were visualized by spraying with a freshly prepared 1:1 mixture of 1% FeCl3 and 1% K3Fe(CN)6. Some sugars, including sucrose, glucose, mannose, fructose, arabinose, xylose, and ribose, and some amino acids, including cysteine, asparagine, histidine, proline, valine, tyrosine, and tryptophan, also gave a color with the reagent. In the experiment, the researchers used many compounds, for example, 1,2-Dihydro-4-methyl-3,6-pyridazinedione (cas: 5754-18-7Quality Control of 1,2-Dihydro-4-methyl-3,6-pyridazinedione).
1,2-Dihydro-4-methyl-3,6-pyridazinedione (cas: 5754-18-7) belongs to pyridazine derivatives. Pyridazine-based compounds continued to be a great source of biologically active compounds as evidenced by the number of publications which emerged in 2021. Pyridazine and derivatives coordinate readily with transition metals to form complexes and catalysts with synthetic utility.Quality Control of 1,2-Dihydro-4-methyl-3,6-pyridazinedione
Referemce:
Pyridazine – Wikipedia,
Pyridazine | C4H4N2 – PubChem