Chemistry is an experimental science, COA of Formula: C5H4N2O2, and the best way to enjoy it and learn about it is performing experiments.Introducing a new discovery about 50681-25-9, Name is 4-Pyridazinecarboxylic Acid, molecular formula is C5H4N2O2, belongs to pyridazines compound. In a document, author is Galeta, Juraj.
A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging
Fluorescent probes that light-up upon reaction with complementary bioorthogonal reagents are superior tools for no-wash fluorogenic bioimaging applications. In this work, a thorough study is presented on a set of seventeen structurally diverse coumarin-tetrazine probes that produce fluorescent dyes with exceptional turn-on ratios when reacted withtrans-cyclooctene (TCO) and bicyclononyne (BCN) dienophiles. In general, formation of the fully aromatic pyridazine-containing dyes resulting from the reaction with BCN was found superior in terms of fluorogenicity. However, evaluation of the probes in cellular imaging experiments revealed that other factors, such as reaction kinetics and good cell permeability, prevail over the fluorescence turn-on properties. The best compound identified in this study showed excellent performance in live cell-labeling experiments and enabled no-wash fluorogenic imaging on a timescale of seconds.
Sometimes chemists are able to propose two or more mechanisms that are consistent with the available data. If a proposed mechanism predicts the wrong experimental rate law, however, the mechanism must be incorrect.Welcome to check out more blogs about 50681-25-9, in my other articles. COA of Formula: C5H4N2O2.
Reference:
Pyridazine – Wikipedia,
,Pyridazine | C4H4N2 – PubChem