Steadman, David et al. published their research in Journal of Medicinal Chemistry in 2022 | CAS: 33050-32-7

6-Chloro-[1,2,4]triazolo[4,3-b]pyridazin-3(2H)-one (cas: 33050-32-7) belongs to pyridazine derivatives. Pyridazines is a six-membered nitrogen-containing significant heterocycle. It has received considerable interest because of its useful applications as natural products, pharmaceuticals, and various bioactive molecules. Pyridazine compounds have attracted interest in various fields like medicinal, industrial, and agricultural research as they are used for numerous biological activities and other applications.Formula: C5H3ClN4O

Virtual Screening Directly Identifies New Fragment-Sized Inhibitors of Carboxylesterase Notum with Nanomolar Activity was written by Steadman, David;Atkinson, Benjamin N.;Zhao, Yuguang;Willis, Nicky J.;Frew, Sarah;Monaghan, Amy;Patel, Chandni;Armstrong, Emma;Costelloe, Kathryn;Magno, Lorenza;Bictash, Magda;Jones, E. Yvonne;Fish, Paul V.;Svensson, Fredrik. And the article was included in Journal of Medicinal Chemistry in 2022.Formula: C5H3ClN4O This article mentions the following:

Notum is a neg. regulator of Wnt signaling acting through the hydrolysis of a palmitoleoylate ester, which is required for Wnt activity. Inhibitors of Notum could be of use in diseases where dysfunctional Notum activity is an underlying cause. A docking-based virtual screen (VS) of a large com. library was used to short-list 952 compounds for exptl. validation as inhibitors of Notum. The VS was successful with 31 compounds having an IC50 < 500 nM. A critical selection process was then applied with two clusters and two singletons selected for hit validation. Optimization of I guided by structural biol. identified potent inhibitors of Notum activity that restored Wnt/β-catenin signaling in cell-based models. The [1,2,4]triazolo[4,3-b]pyridazin-3(2H)-one series represent a new chem. class of Notum inhibitors and the first to be discovered by a VS campaign. These results demonstrate the value of VS with well-designed docking models based on X-ray structures. In the experiment, the researchers used many compounds, for example, 6-Chloro-[1,2,4]triazolo[4,3-b]pyridazin-3(2H)-one (cas: 33050-32-7Formula: C5H3ClN4O).

6-Chloro-[1,2,4]triazolo[4,3-b]pyridazin-3(2H)-one (cas: 33050-32-7) belongs to pyridazine derivatives. Pyridazines is a six-membered nitrogen-containing significant heterocycle. It has received considerable interest because of its useful applications as natural products, pharmaceuticals, and various bioactive molecules. Pyridazine compounds have attracted interest in various fields like medicinal, industrial, and agricultural research as they are used for numerous biological activities and other applications.Formula: C5H3ClN4O

Referemce:
Pyridazine – Wikipedia,
Pyridazine | C4H4N2 – PubChem