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Purification and Some Properties of Nicotinate Phosphoribosyltransferase from Hog Liver
Purification of nicotinate phosphoribosyltransferase (NPRTase, EC 2.4.2.11) from hog liver and some of its properties were investigated.Purified enzyme was found to be homogeneous by the criterion of polyacrylamide gel disc electrophoresis and to have a molecular weight of about 120,000.The subunit molecular weight was found to be 64,000, using SDS polyacrylamide gel disc electrophoresis.The optimum pH and isoelectric point of the enzyme were 7.3-7.4 and 4.8, respectively.Mn(2+), Co(2+), and Mg(2+) were effective in meeting the NPRTase requirement for a divalent cation.ATP had a stimulative effect on the enzyme activity in the presence of Mg(2+), on the other hand, in the presence of Mn(2+), ATP had an inhibitory effect on the enzyme activity.Of the purine nucleotides examined, only ATP stimulated NPRTase activity, and GTP did not.On the contrary, all other nucleotides inhibited the activity.Nicotinate mononucleotide, which is a reaction product, inhibited NPRTase activity.Among the nicotinic acid analogues tested, only pyrazine-2-carboxylic acid inhibited the activity.Nicotinamide, quinolinic acid, adenine, and hypoxanthine were not phosphoribosylated by NPRTase.Incubation of NPRTase with SH-modifying reagents caused loss of NPRTase activity.
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Pyridazine – Wikipedia,
Pyridazine | C4H4N482 – PubChem